|
α-smooth muscle actin (SMA)
|
Smooth muscle or myofibroblastic differentiation
|
|
α-1 antichymotrypsin
|
Outdated histiocytic marker
|
|
α-1 antitrypsin
|
α-1 antitrypsin deficiency (liver tissue)
|
|
ACTH
|
Pituitary tumors
|
|
Actin, smooth muscle (SMA)
|
Smooth muscle or myofibroblastic differentiation
|
|
Actin, muscle-specific (MSA) (HHF-35)
|
Smooth muscle and skeletal muscle differentiation
|
|
Adenovirus
|
Adenovirus infection
|
|
AFP
|
Hepatoma, Hepatoid Carcinoma, Yolk sac tumors
|
|
ALK-1
|
ALK-1 positive lymphoma
|
|
AMACR (P504S)
|
Prostate cancer diagnosis, also other carcinomas
|
|
Amebiasis (E. Histolytica)
|
Ambeiasis – cross reacts with histiocytes, so order CD68 with it
|
|
Amyloid AA protein
|
Amyloid subtyping
|
|
Amyloid P protein
|
Amyloid subtyping (also stains normal elastic tissue)
|
|
Androgen Receptor
|
Metastatic carcinoma unknown primary
|
|
Annexin A1
|
Hairy cell leukemia, T-cells, myeloid cells
|
|
AP12 (MALT) (FISH)
|
t(11:18) (q21;q21)
|
|
Arginase-1
|
Hepatocellular carcinoma, hepatoid differentiation
|
|
b-catenin
|
Fibromatosis, metastatic carcinoma unknown primary
|
|
BAF47 (INI1)
|
Epithelioid sarcoma, teratoid/rhabdoid tumor, MPNST
|
|
Bartonella Henselae
|
Cat scratch disease organism
|
|
B72.3
|
Mesothelioma vs. adenocarcinoma
|
|
BCL-1
|
Same as Cyclin D1
|
|
BCL-2
|
Lymphoma typing, soft tissue tumors
|
|
BCL-6
|
Lymphoma typing
|
|
Ber-EP4
|
Mesothelioma vs. adenocarcinoma
|
|
BG-8
|
Mesothelioma vs. adenocarcinoma
|
|
BK virus
|
Marker of BK Polyomavirus infection
|
|
Bob.1
|
B-cells, plasma cells
|
|
Brachyury
|
Chordoma, Hemangioblastoma
|
|
BRST-1 (BCA-225)
|
Some epithelial tumors
|
|
c-MYC probes (FISH)
|
Burkitt lymphoma
|
|
C4d
|
Used for assessment of rejection in transplant bxs
|
|
C9
|
Diagnosis of myocardial infarction (used with troponin T)
|
|
c-kit (CD117)
|
Gastrointestinal stromal tumors, mast cells
|
|
CA-125
|
Multiple epithelial tumors, also in mesothelial cells
|
|
CA19-9
|
Metastatic carcinoma unknown primary
|
|
Calcitonin
|
Medullary carcinoma thyroid
|
|
Caldesmon
|
Smooth muscle differentiation
|
|
Calponin
|
Smooth muscle differentiation, myoepithelial cells
|
|
Calretinin
|
Mesothelioma, adrenal cortical tumors, sex cord stromal
tumors
|
|
Cat Scratch Disease
|
Bartonella henselae infection
|
|
Cathepsin K
|
TFEB translocation-associated renal cell carcinomas
|
|
CD1a
|
Thymic T-cells, thymoma, Langerhans cells
|
|
CD2 T-cells)
|
Lymphoma/leukemia typing
|
|
CD3 (T-cells)
|
Lymphoma/leukemia typing
|
|
CD4 (T-helper, inducer)
|
Lymphoma/leukemia typing
|
|
CD5 (T-cells)
|
Lymphoma/leukemia typing (damaged by B5)
|
|
CD7 (T-cells)
|
Lymphoma/leukemia typing
|
|
CD8 (T-suppressor/cytotoxic)
|
Lymphoma/leukemia typing
|
|
CD10 (CALLA)
|
Lymphoma typing, DFSP vs DF, endometrial stromal tumors
|
|
CD11c
|
Leukemia typing, hairy cell leukemia
|
|
CD13
|
Leukemia typing, myeloid marker
|
|
CD15
|
Lymphoma typing, mesothelioma vs. adenocarcinoma
|
|
CD20 (L26)
|
Lymphoma/leukemia typing
|
|
CD21
|
Follicular dendritic cells, lymphoma typing
|
|
CD23
|
Lymphoma/leukemia typing
|
|
CD25 (IL-2)
|
Lymphoma/leukemia typing
|
|
CD30 (Ki-1)
|
Lymphoma typing
|
|
CD31
|
Vascular differentiation, some histiocytes
|
|
CD33
|
Monocytic marker
|
|
CD34
|
Soft tissue tumor classification, leukemia typing
|
|
CD38
|
Plasma cell differentiation (does not work well on B5)
|
|
CD43 (MT1)
|
Lymphoma/leukemia typing
|
|
CD44
|
Burkitt vs non-Burkitt, carcinoid prognosis
|
|
CD45 (LCA)
|
Recognition of lymphohematopoetic tumors
|
|
CD45RA (4KB5)
|
Lymphoma/ leukemia typing
|
|
CD45RB
|
Lymphoma/ leukemia typing
|
|
CD45RO (UCHL1)
|
Lymphoma typing (T-cells, some myeloid cells)
|
|
CD45RO (OPD4)
|
Lymphoma typing
|
|
CD56 (NCAM)
|
Lymphoma typing, neuroendocrine differentiation
|
|
CD57 (NK-1, Leu 7)
|
Lymphoma typing, neuroendocrine differentiation
|
|
CD61
|
Megakaryocytes, megakaryocytic leukemia
|
|
CD68 (KP1)
|
Histiocytic/myeloid/monocytic marker
|
|
CD68 (PG-M1)
|
Histiocytic/monocytic marker
|
|
CD71 (transferrin receptor)
|
Erythroid cells, some lymphomas
|
|
CD74 (LN-2, B-cells)
|
Lymphoma typing
|
|
CD79a (B-cells)
|
Lymphoma/leukemia typing
|
|
CD82 (KAI-1)
|
Kidney tumor typing
|
|
CD99 (MIC-2)
|
Soft tissue tumor classification
|
|
CD117 (c-kit)
|
Gastrointestinal stromal tumors, mast cells, blasts
|
|
CD123
|
Lymphoma typing – plasmacytoid dendritic cell tumors
|
|
CD138
|
Plasma cells (also stains many epithelial tumors)
|
| CD 163 |
Histiocytic - Monocytic marker |
|
CDK4
|
Liposarcoma
|
|
CDX2
|
Metastatic carcinoma unknown primary
|
|
CEA (clone COL-1)
|
Mesothelioma vs. adenocarcinoma, metastatic ca. unknown primary
|
|
CEA (polyclonal)
|
Hepatoma vs. adenocarcinoma (use COL1 for other applications)
|
|
c-erbB2 (HER2)
|
HER2 overexpression
|
|
Chromogranin
|
Neuroendocrine differentiation
|
|
Claudin-1
|
Perineurioma
|
|
Claudin-5
|
Endothelial marker
|
|
Clusterin
|
Lymphoma typing, follicular dendritic marker
|
|
CMV
|
Cytomegalovirus infection
|
|
Coccidioidomycosis
|
Coccidiodomyosis infection (cross reacts w Histoplasmosis)
|
|
Collagen IV
|
Soft tissue tumor classification
|
|
Cryptococcus
|
Crytococcus neoformans infection
|
|
Cyclin D1
|
Mantle cell lymphoma
|
|
Cytokeratin 5
|
Mesothelioma, squamous carcinoma
|
|
Cytokeratin 7
|
Metastatic carcinoma unknown primary
|
|
Cytokeratin 14
|
Oncocytic tumors
|
|
Cytokeratin 15
|
Useful for diagnosis of cutaneous adnexal tumors
|
|
Cytokeratin 17
|
Metastatic carcinoma unknown primary
|
|
Cytokeratin 19
|
Papillary thyroid carcinoma, prognostic marker in islet cell tumors
|
|
Cytokeratin 20
|
Metastatic carcinoma unknown primary
|
|
Cytokeratin AE1/AE3
|
Epithelial tumors, hepatoma vs. adenocarcinoma (NOT
a “Pan-CK”)
|
|
Cytokeratin HMW (34bE12)
|
Metastatic carcinoma unknown primary, prostate basal cells
|
|
Cytokeratin LMW (8,18)
|
Epithelial tumors, especially renal, prostate, hepatoma
|
|
CXCL13
|
Angioimmunoblastic T-cell lymphoma
|
|
D2-40 (podoplanin)
|
Mesothelioma, lymphatics, seminoma
|
|
DBA.44
|
Hairy cell leukemia, some marginal zone lymphomas
|
|
Desmin
|
Smooth and skeletal muscle differentiation
|
|
DOG1
|
Gastrointestinal stromal tumor (GIST) marker (better than
c-kit, CD117)
|
|
EBV (EBER probe)
|
In-situ hybridization stain for EBV
|
|
EBV (LMP-1)
|
Epstein-Barr virus infection
|
|
E-Cadherin
|
Lobular vs. Ductal breast carcinoma, erythroid precursors
|
|
E. Histolytica
|
Amebiasis- cross reacts with histiocytes, so order CD68 with it
|
|
EGFR (pharmDx)
|
Erbitux eligibility (FDA-approved test)
|
|
EGFR (31G7)
|
Sweat gland carcinoma, squamous tumors
|
|
EGFR amplification (FISH)
|
EGFR gene amplification status
|
|
EMA
|
Metastatic carcinoma of unknown primary, Lymphoma typing
|
|
Emerin
|
Useful for highlighting nuclear membrane in papillary ca thyroid
|
|
ERCC1
|
Prognostic marker in non-small cell lung carcinoma
|
|
ERG
|
Endothelial marker (stains nuclei), subset of prostate carcinoma
|
|
Estrogen receptor
|
Breast cancer prognostic marker, metastatic carcinoma
unknown primary
|
|
EWSR1 probe (FISH)
|
Ewing/ PNET, DSRCT, clear cell sarcoma, extraskel
myxoid chondrosarc
|
|
Factor VIII
|
Megakaryocytic marker, endothelial marker
|
|
Factor XIIIa
|
Dermatofibroma vs. DFSP
|
|
Fascin
|
Hodgkin’s cells, other reticulum cells
|
|
FLI-1
|
Ewing’s sarcoma, vascular tumors, lymphoblastic lymphoma
|
|
FSH
|
Pituitary tumors
|
|
FUS probe (FISH)
|
Low grade fibromyxoid sarcoma, angiomatoid MFH,
myxoid liposarc
|
|
Galectin-3
|
Papillary thyroid carcinoma
|
|
Gastrin
|
Gastrinomas
|
|
GCDFP-15
|
Breast carcinoma, sweat gland, salivary gland marker
|
|
GFAP
|
Glial tumors, salivary gland tumors
|
|
GH
|
Pituitary tumors
|
|
Giardia
|
Giardia lamblia infection
|
|
Glucagon
|
Pancreatic endocrine tumors
|
|
GLUT-1
|
Perineurioma
|
|
Glycophorin A
|
Erythroid marker
|
|
Glypican 3
|
Yolk sac tumor, hepatoma, hepatoid adenocarcinoma
|
|
Granzyme B
|
Lymphoma typing
|
|
H. Pylori
|
Helicobacter pylori infection
|
|
HBME-1
|
Chordoma, papillary thyroid carcinoma
|
|
HbsAg
|
Hepatitis B surface antigen
|
|
HCG
|
Choriocarcinoma
|
|
Hemoglobin
|
Erythroid marker
|
|
HER-1
|
Same as EGFR
|
|
HepPar1
|
Hepatoma, gastric carcinoma
|
|
HER2 immunostain
|
HER2 protein overexpression
|
|
HER2 by FISH
|
HER2 gene amplification status
|
|
Herpes Simplex I & II
|
Herpesvirus I and II infection
|
|
HHV8 (KSHV)
|
Kaposi’s sarcoma, some cases Castleman’s, lymphoma
|
|
Histoplasmosis
|
Histoplasmosis capsulatum infection (also stains other fungi)
|
|
HIV (p24-gag)
|
Detects HIV only in tissues that contain follicular dendritic cells
|
|
HLA-Dr (LN3)
|
Leukemia typing
|
|
HLA-G
|
Intermediate trophoblasts (chorioca, other)
|
|
HMB-45
|
Melanoma marker, angiomyolipoma and other PEComas
|
|
HPL
|
Germ cell tumors, trophoblastic tumors
|
|
HPV-Pan
|
In-situ hybridization stain, detects HPV types 6, 11, 16, 18, 31,
33, 35, 51
|
|
HPV, types 6/11
|
In-situ hybridization stain
|
|
HPV, types 16/18
|
In-situ hybridization stain
|
|
HPV, types 31/33/51
|
In-situ hybridization stain
|
|
IgA
|
Plasma cell subclassification
|
|
IgG
|
Plasma cell subclassification
|
|
IgG4
|
IgG4-related sclerosing diseases
|
|
IgM
|
Plasma cell subclassification
|
|
IgH-MALT1 (MALT) (FISH)
|
t(14;18) (q32;q21)
|
|
IL-2 (CD25)
|
Lymphoma/leukemia typing
|
|
IMP3 (L523S, KOC)
|
Dysplasia marker, marker of serous carcinoma
|
|
Inhibin
|
Adrenal cortical tumors, sex-cord stromal tumors
|
|
INI-1 (BAF47)
|
Epithelioid sarcoma, teratoid/rhabdoid tumor, MPNST
|
|
Insulin
|
Pancreatic endocrine tumors
|
|
Kappa light chains
|
Plasma cell clonality
|
|
Ki-67 (clone K2)
|
Proliferative fraction, viable fat cell membranes, lipoblasts
|
|
Ki-67 (clone MIB-1)
|
Proliferative fraction, hyalinizing trabecular tumor of thyroid
|
|
Kit
|
Same as c-kit, CD117
|
|
Ki-67 (clone SP6)
|
Proliferative fraction
|
|
KOC (L523S, IMP3)
|
Dysplasia marker, marker of serous ca
|
|
L523S (KOC, IMP3)
|
Dysplasia marker, marker of serous ca
|
|
Lambda light chains
|
Plasma cell clonality
|
|
Laminin
|
Basement membrane marker
|
|
LAT
|
T-cells
|
|
LCA (CD45)
|
Lymphohematopoetic tumors
|
| LEF-1 |
Marker of SLL/CLL |
|
LH
|
Pituitary tumors
|
|
Lipase
|
Acinar cell tumor of pancreas, other
|
|
LN3 (HLA-Dr)
|
Leukemia typing
|
|
Lysozyme
|
Myeloid and histiocytic marker
|
|
MALT probes (FISH)
|
MALT lymphomas, 3 probes, API2/MALT1 t(1118)
(q21q21), IgH/MALT1 t(1418), and BCL6 breakapart
probe
|
|
Mammaglobin
|
Breast marker, salivary glands, sweat glands
|
|
MART-1 (clone A103)
|
Melanoma, adrenal cortical tumors, sex cord-stromal tumors,
PEComas
|
|
MART-1 (M2-7C10+M29E3)
|
Melanoma, angiomyolipoma and other PEComas
|
|
Mast cell tryptase
|
Mastocytosis
|
|
MDM2
|
Liposarcoma
|
|
Mesothelin (clone 5B2)
|
Mesothelioma, serous carcinoma, squamous carcinoma
|
|
MIC-2 (CD99)
|
Soft tissue tumor classification
|
|
MiTF
|
Melanoma, thyroid tumors, many soft tissue tumors
|
|
Mitochondria
|
Oncocytic tumors
|
|
MLH1
|
Hereditary cancer syndromes, mismatch repair gene mutations
|
|
MOC-31
|
Mesothelioma vs. adenocarcinoma
|
|
MSA (muscle-specific actin)
|
HHF-35, smooth muscle and skeletal muscle
|
|
MSH2
|
Hereditary colon cancer syndrome, mismatch repair
gene mutations
|
|
MSH6
|
Hereditary colon cancer syndrome, mismatch repair
gene mutations
|
|
MUC1
|
Paget’s disease
|
|
MUC2
|
Ovarian mucinous carcinoma vs. colorectal carcinoma
|
|
MUC4
|
Marker of low grade fibromyxoid sarcoma
|
|
MUC5AC
|
Ovarian mucinous carcinoma vs. colorectal carcinoma
|
|
MUC6
|
Seminal vesicle marker, serous neoplasms of pancreas
|
|
MUM1
|
Lymphoma typing
|
| MYC Protein IHC |
Assists in diagnosis of Burkitt lymphoma |
|
Mycobacteria
|
Mycobacteria (typical and atypical) (vendor has D/C’d antibody)
|
|
MYC probes (FISH)
|
Burkitt lymphoma
|
|
Myeloperoxidase
|
Myeloid and histiocytic marker
|
|
Myogenin
|
Rhabdomyosarcoma
|
| Myosin Heavy Chain |
Smooth muscle and myeopithelial marker |
|
Myosin, smooth muscle
|
Myoepithelial marker, smooth muscle differentiation
|
|
N-Cadherin
|
Metastatic carcinoma unknown primary
|
|
Napsin A
|
Lung carcinoma, alveolar macrophages, some renal cell carcinomas
|
|
NeuN
|
Neuronal marker
|
|
Neurofilament
|
Neural tumors, occasional non-neural tumors
|
|
NGFR
|
Soft tissue tumor classification
|
|
NKI-C3
|
Melanoma, neural marker, lysosomal marker
|
|
NPM
|
AML subset
|
|
NKX3.1
|
Sensitive and specific nuclear marker of prostate carcinoma
|
|
NSE
|
Worthless marker, don’t use it
|
|
NUT
|
Marker of NUT positive midline carcinomas
|
|
Oct2
|
Lymphoma typing
|
|
OCT3/4
|
Seminoma, embryonal carcinoma
|
|
Osteonectin
|
Osteosarcoma, also other sarcomas (not bone-specific)
|
|
p16 (INK4)
|
Cervical dysplasia, HPV-associated head and neck squamous
carcinomas
|
|
P24-gag
|
Detects HIV only in tissues that contain follicular dendritic cells
|
|
P501S (Prostein)
|
Prostate marker
|
|
P504S (AMACR)
|
Prostate cancer diagnosis
|
|
P504S / p63 cocktail
|
Prostate cancer diagnosis
|
|
p53
|
p53 tumor suppressor gene mutations
|
|
p57
|
Molar pregnancy (in conjunction with Ki-67)
|
|
p63
|
Myoepithelial, prostatic basal cell, squamous marker
|
|
P63/ P504S cocktail
|
Prostate cancer diagnosis
|
|
Pancreatic polypeptide
|
Pancreatic endocrine tumors
|
|
Parathyroid hormone (PTH)
|
Parathyroid tumors
|
|
Parvovirus
|
Parvovirus B19 infection
|
|
Parathyroid hormone (PTH)
|
Parathyroid tumors
|
|
Parvovirus
|
Parvovirus B19 infection
|
|
Pax-2
|
Renal cell carcinoma, serous carcinoma, lymphoid cells
|
|
Pax-5 (B-cells)
|
B-cells, some neuroendocrine tumors
|
|
Pax-8
|
Kidney, thyroid, female genital tract carcinomas, some
lymphoid cells
|
|
PCP (Pneumocystis carinii)
|
Pneumocystis carinii infection
|
| PD-1 |
Assist in dx of angioimmunoblastic lymphoma |
|
PDEGF
|
Platelet-derived epidermal growth factor
|
|
PDGFR-alpha
|
Diagnosis of c-kit negative GISTs (DOG1 is better for this)
|
| PDX1 |
Pancreatic tumors |
|
Perforin
|
Lymphoma typing
|
|
PE-10 (SP-A)
|
Lung adenocarcinoma and some thyroid carcinomas
|
|
PGP9.5
|
Neural/neuroendocrine marker
|
|
PHH3
|
Mitosis marker
|
|
Placental S100 (S100P)
|
Benign vs malignant pancreatic lesions, urothelial ca, other
|
|
PLAP
|
Many carcinomas, also some germ cell tumors
|
|
PMS2
|
Hereditary colon cancer syndrome, mismatch repair gene
mutations
|
|
Pneumocystis (PCP)
|
Pneumocystis carinii infection
|
|
Podoplanin (D2-40)
|
Same as D2-40, mesothelioma, lymphatics, seminoma
|
|
Polyomavirus (BK, SV-40)
|
Marker of BK Polyomavirus infection
|
|
Prealbumin (transthyretin)
|
Choroid plexus tumors, some types of amyloid
|
|
Procollagen 1
|
Fibroblastic differentiation, AFX, also some carcinomas
|
|
ProExC
|
Cervical dysplasia
|
|
Progesterone receptor
|
Breast cancer prognostic marker
|
|
Prolactin
|
Pituitary tumors
|
|
Prostein (P501S)
|
Prostate marker
|
|
pS2
|
Breast prognostic marker
|
|
PSA (prostate)
|
Prostate marker
|
|
PSAP (prostate)
|
Prostate marker (prostatic acid phos), also rectal carcinoid
tumors
|
|
PSMA (prostate)
|
Prostate marker
|
|
PTH
|
Parathyroid tumors
|
|
pVHL
|
Renal tumors, clear cell ca fem gen tract, bile duct marker
|
|
RCC (PN-15)
|
Renal cell carcinoma marker (Pax8 is much more sensitive)
|
|
Ret oncoprotein
|
Ganglion cells
|
|
RRM2
|
Evaluation of urothelial dysplasia
|
|
RSV
|
Respiratory Synctitial Virus
|
|
Rocky Mountain Spotted Fever
|
Rocky Mountain Spotted Fever bug
|
|
S100
|
Melanoma, neural marker, myoepithelial, other
|
|
S100P (Placental S100)
|
Benign vs malignant pancreatic lesions, urothelial ca, other
|
|
SALL4
|
Germ cell tumors, hepatoid adenocarcinoma of stomach
|
|
SMA (smooth muscle actin)
|
Smooth muscle or myofibroblastic differentiation
|
|
Smooth muscle myosin
|
Myoepithelial marker, smooth muscle differentiation
|
|
Smoothelin
|
Used to distinguish muscularis mucosa from detrusor in
bladder bxs
|
|
Somatostatin
|
Pancreatic endocrine tumors
|
|
Sox10
|
Sensitive melanoma marker (better than S100), neural,
myoepithelial
|
|
SV-40 (BK, Polyomavirus)
|
Marker of BK Polyomavirus infection
|
|
Synaptophysin
|
Neuroendocrine differentiation
|
|
Syphilis
|
Treponema pallidum infection
|
|
SYT probe, t(X;18) (FISH)
|
Synovial sarcoma
|
|
t(11;14)
|
Mantle cell lymphoma
|
|
t(14;16)
|
Follicular lymphoma
|
|
Tau protein
|
Alzheimer’s disease
|
|
TDT
|
Lymphoma/leukemia typing, Merkel cell tumors
|
|
Telomerase
|
Identification of cells with telomerase activity
|
|
TFE3
|
Alveolar soft part sarcoma, some renal tumors
|
|
Thrombomodulin
|
Urothelial carcinoma, mesothelioma, squamous tumors
|
|
Thyroglobulin
|
Thyroid carcinoma
|
|
TIA-1 (cytotoxic)
|
Lymphoma typing
|
|
TLE-1
|
Synovial sarcoma, other tumors
|
|
Topoisomerase
|
Prognostic marker
|
|
Transthyretin (prealbumin)
|
Choroid plexus tumors, some types of amyloid
|
|
TRAP
|
Hairy cell leukemia
|
|
Troponin T
|
Diagnosis of myocardial infarction (used with C9)
|
|
Trypsin
|
Acinar cell tumor of pancreas
|
|
Tryptase, mast cell
|
Mast cell marker
|
|
TSH
|
Pituitary tumors
|
|
TTF-1
|
Lung & thyroid marker, also some neuroendocrine tumors
|
|
Tyrosinase
|
Melanoma maker
|
|
Ubiquitin
|
Alzheimer's disease, recognition of Mallory bodies in liver
|
|
Urinary FISH panel (FISH on urine)
|
Cytologic diagnosis of bladder cancer
|
|
Varicella Zoster
|
Varicella Zoster infection
|
|
VEGF
|
Misc tumors
|
|
Villin
|
Metastatic carcinoma unknown primary
|
|
Vimentin
|
Metastatic carcinoma unknown primary
|
|
VIP
|
Pancreatic endocrine tumors
|
|
VS38
|
Plasma cells, plasmacytoid, epithelial cells
|
|
Whipple's disease
|
Whipple's disease
|
|
WT-1 (6F-H2)
|
Mesothelioma and serous carcinoma marker
|
|
ZAP-70
|
CLL prognostication
|
